Ultrastructural analysis of survival in cultured smooth muscle cells isolated from stroke-prone spontaneously hypertensive rats: effect of growth factors

MotokiTagami; KazuoYamagata; YasuoNaraHideakiFujinoAkiyoshiKubotaFujioNumanoYukioYamori

首页> 外文期刊>cell and tissue research >Ultrastructural analysis of survival in cultured smooth muscle cells isolated from stroke-prone spontaneously hypertensive rats: effect of growth factors

【24h】

Ultrastructural analysis of survival in cultured smooth muscle cells isolated from stroke-prone spontaneously hypertensive rats: effect of growth factors

机译：Ultrastructural analysis of survival in cultured smooth muscle cells isolated from stroke-prone spontaneously hypertensive rats: effect of growth factors

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Our previous study in vivo suggested that vascular smooth muscle cells (VSMCs) in stroke-prone spontaneously hypertensive rats (SHRSP) were vulnerable when plasma components were deficient. Therefore, we cultured VSMCs isolated from normotensive and hypertensive rats to clarify the weakness of VSMCs isolated from hypertensive rats and maintained in plasma-deficient conditions by employing ultrastructural and biochemical analyses. VSMCs, obtained from normotensive rats and cultured without fetal bovine serum (FBS) for 1 week, were intact and well differentiated; without FBS for 2 weeks retained their original structures except for several degenerative changes. VSMCs, obtained from hypertensive rats and cultured without FBS for 2 weeks, were extensively damaged and lost their cell organelles. Apoptotic bodies were frequently observed. We also cultured VSMCs in medium containing a variety of growth factors. VSMCs obtained from normotensive rats and cultured with epidermal growth factor or insulin-like growth factor-1 for 2 weeks were almost intact, as were VSMCs from hypertensive rats, although some degenerative changes of cell organelles were observed. VSMCs from hypertensive rats, maintained with platelet-derived growth factor-BB or basic fibroblast growth factor, were generally in poor condition. Thus VSMCs from hypertensive rats have hereditary weaknesses in cell survival including apoptosis and require specific growth factors for their maintenance.

著录项

来源
《cell and tissue research》 |1996年第2期|205-215|共页
作者
MotokiTagami; KazuoYamagata; YasuoNaraHideakiFujinoAkiyoshiKubotaFujioNumanoYukioYamori;
展开▼
作者单位

Department of Internal Medicine, SANRAKU Hospital, Chiyoda-ku, Tokyo 101, Japan;

Sumitomo Metal Industry, Chiyoda-ku, Tokyo 100, Japan;

Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606, JapanThe Third Department of Internal Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113, Japan;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类
关键词

Ultrastructural analysis of survival in cultured smooth muscle cells isolated from stroke-prone spontaneously hypertensive rats: effect of growth factors

摘要

著录项

相关主题

期刊订阅