首页>
外文期刊>applied microbiology and biotechnology
>Electrophoretic karyotyping fromTolypocladium inflatumand six related strains allows differentiation of morpologically similar species
【24h】
Electrophoretic karyotyping fromTolypocladium inflatumand six related strains allows differentiation of morpologically similar species
展开▼
机译：Electrophoretic karyotyping fromTolypocladium inflatumand six related strains allows differentiation of morpologically similar species
The electropheretic karyotype for the imperfect filamentous fungusTolypocladium inflatumand for six related strains is presented. Pulsed-field gel electrophoresis was used with improved separation conditions to separate DNA from 6.6 Mb to 1.05 Mb in size. Using probes encoding rRNA or the β-tubulin gene fromT.iinflatumthe corresponding genes were detected on designated chromosomes. In addition, two recombinant lambda clones, carryingT. inflatumchromosomal DNA, were used as chromosome-specific probes. Although all strains investigated are very similar in their morphology, significant chromosome-lengt polymorphisms were detected, allowing easy strain differentiation. The polymorphism was confirmed using an rRNA probe for genomic mapping. All strains contain a homologous “minichromosome” of 1.05 Mb. Finally, the resolution of very large DNA enabled us to separate a 6.6-Mb DNA band that specifically hybridizes with mitochondrial gene probes. The electrophoretic karyotyping presented here may be regarded as a reliable molecular tool to differentiate morphologically very similar filamentous f
展开▼
