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>Rat vascular smooth muscle cells immortalized with SV40 large T antigen possess defined smooth muscle cell characteristics including growth inhibition by heparin
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Rat vascular smooth muscle cells immortalized with SV40 large T antigen possess defined smooth muscle cell characteristics including growth inhibition by heparin
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机译：Rat vascular smooth muscle cells immortalized with SV40 large T antigen possess defined smooth muscle cell characteristics including growth inhibition by heparin
AbstractRat aortic smooth muscle cells (SMC) have been established by retroviral delivery of the complementary DNA (cDNA) for the simian virus 40 large T antigen (SV40LT) and examined for SMC phenotypic markers and growth characteristics, including responsiveness to the antiproliferative effects of heparin. The transfected cells (SV40LT–SMC) maintain defined SMC characteristics for more than 215 population doublings (PD) as judged by muscle‐specific actin expression and growth inhibition by heparin. SV40LT–SMC>129 PD become transformed while SV40LT–SMC80 PD) SV40LT–SMC proliferation. The antiproliferative effects of heparin are similar to those previously observed for SMC by several criteria; the dose‐response inhibition curves are indistinguishable from those obtained with nontransfected cells, other glycosaminoglycans have little effect on SV40LT–SMC growth, the antiproliferative effects of heparin are reversed in the presence of epidermal growth factor, and heparin displays high‐affinity saturable binding to SV40LT–SMC. In conclusion, SV40LT–SMC are a continuous line of SMC‐like cells that are sensitive to the growth inhibitor, heparin. SV40LT–SMC should facilitate studies of heparin inhibition and may be applicable for the st
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