Transfer of transformed plastids of Lesquerella fendleri to Brassica napus plants by methods of somatic hybridization is carried out. Transplastomic plants of L. fendleri which contain the selective marker gene aadA16gfp in the plastid DNA are used in the experiments. This gene confers resistance to spectomy-cin/streptomycin and green fluorescence in ultraviolet radiation. Protoplasts of chlorophyll-deficient plants of B. napus are fused with gamma-irradiated protoplasts of transplastomic plants of L. fendleri. The green colonies which grow in the selection medium with spectinomycin and streptomycin were selected as hybrids. Regeneration of the shoots was observed for two of the 59 hybrid colonies. Morphologically normal plants were obtained for a single line. Molecular biological analysis of the plants demonstrated that they are transplastomic hybrids that possess the nuclear genome of B. napus and the transformed plastome of L. fendleri.
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