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首页> 外文期刊>journal of applied toxicology >Evaluation of the developmental toxicity of five compounds with the frog embryo teratogenesis assay:Xenopus(FETAX) and a metabolic activation system
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Evaluation of the developmental toxicity of five compounds with the frog embryo teratogenesis assay:Xenopus(FETAX) and a metabolic activation system

机译：Evaluation of the developmental toxicity of five compounds with the frog embryo teratogenesis assay:Xenopus(FETAX) and a metabolic activation system

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AbstractThe potential teratogenic hazard of five compounds was evaluated using the Frog Embryo Teratogenesis Assay—Xenopus(FETAX) and a metabolic activation system. Embryos of the South African clawed frog,Xenopus laevis, were exposed to (i) three compounds suspected to be proteratogenic in mammalian test systems—2‐acetylaminofluorene (2‐AAF), rifampicin (RA) and benzoapyrene (BP)for 96 h; (ii) one compound unaffected by mixed‐functional oxidase (MFO) metabolism—ZnSO4; (iii) one compound thought to be inactivated by cytochrome P‐450—cytochalasin D (CD). Two separate static renewal tests were conducted with and without the presence of an exogenous metabolic activation system (MAS). The metabolic activation system consisted of Aroclor 1254‐induced rat liver microsomes. The teratogenic potential of each compound and the effects of metabolic activation were based on teratogenic indices TI=96 hLC50/96 hEC50(malformation), types and severity of malformation, and effects on embryo growth. Metabolic activation increased the potential teratogenic hazard of 2‐AAF, RA and BP byTIfactors of 1.3, 2.8 and 6.8, respectively. The teratogenic potential of ZnSO4was virtually unaffected by the MAS. The MAS significantly reduced the teratogenic potential of CD by aTIfactor of 2.7. These results demonstrate the utility and importance of a MAS forin vitrodevelopmental toxicity screens such as FETAX. Consistent use of a MAS with FETAX should reduce the number of potential false‐positive and false

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