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>Studies on Cellular Adhesion ofXenopus laevisMelanophores: Modulation of Cell‐Cell and Cell‐Substratum Adhesion In Vitro by EndogenousXenopusGalactoside‐Binding Lectin
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Studies on Cellular Adhesion ofXenopus laevisMelanophores: Modulation of Cell‐Cell and Cell‐Substratum Adhesion In Vitro by EndogenousXenopusGalactoside‐Binding Lectin
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机译：Studies on Cellular Adhesion ofXenopus laevisMelanophores: Modulation of Cell‐Cell and Cell‐Substratum Adhesion In Vitro by EndogenousXenopusGalactoside‐Binding Lectin
We have investigated cell‐cell and cell‐substratum adhesion ofXenopus laevisneural crest cells at various stages of melanophore differentiation. Single‐cell suspensions were obtained by trypsinization and aggregated in a cell‐cell adhesion assay. Unpigmented cells did not adhere while the rate of adhesion of melanophores correlated with the degree of melanization. Melanophore cell‐cell adhesion decreased significantly in the presence of beta‐galactosidase, which suggests that cell‐surface galactose is involved. Beta‐galactoside‐binding lectin has been isolated and purified from embryos at the stage of neural crest migration. When added to aggregating cells smaller, looser clusters formed compared to controls. When lectin was added to cells in stationary culture to test cell‐substratum adhesion, melanophores spread more smoothly and formed more regular spacing patterns. These results suggest that this lectin can modulate receptors used in cell‐cell and cell‐substratum a
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