...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>journal of cellular biochemistry >Purification and stability characterization of a cell regulatory sialoglgycopeptide inhibitor
【24h】

Purification and stability characterization of a cell regulatory sialoglgycopeptide inhibitor

机译：Purification and stability characterization of a cell regulatory sialoglgycopeptide inhibitor

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

AbstractPrevious attempts to physically separate the cell cycle inhibitory and protease in activities in preparations of a purified cell regulatory sialoglycopeptide (CeReS) inhibitor were largely unsuccessful. Gradient elution of the inhibitor preparation from a DEAE HPLC column separated the cell growth inhibitor from the protease, and the two activities have been shown to be distinct and non‐overlapping. The additional purification increased the specific biological activity of the CeReS preparation by approximately two‐fold. The major inhibitory fraction that eluted from the DEAE column was further analyzed by tricine‐SDS‐PAGE and microbore reverse phase HPLC and shown to be homogeneous in nature. Two other fractions separated by DEAE HPLC, also devoid of protease activity, were shown to be inhibitory to cell proliferation and most likely represented modified relatives of the CeReS inhibitor. The highly purified CeReS was chemically characterized for amino acid and carbohydrate composition and the role of the carbohydrate in cell proliferation inhibition, stability, and protease resistance was assessed. © 1995 Wiley

著录项

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号