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首页> 外文期刊>Veterinary Research: A Journal on Animal Infection >Effect of field capture on the measurement of cellular immune responses in wild ferrets (Mustela furo), vectors of bovine tuberculosis in New Zealand.
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Effect of field capture on the measurement of cellular immune responses in wild ferrets (Mustela furo), vectors of bovine tuberculosis in New Zealand.

机译：Effect of field capture on the measurement of cellular immune responses in wild ferrets (Mustela furo), vectors of bovine tuberculosis in New Zealand.

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In vitro lymphocyte reactivity and levels of physiological stress were measured in wild-caught ferrets. The results were compared to those observed in laboratory-maintained domesticated ferrets. Over 12 months, 80 ferrets were live-captured from a tuberculosis (Tb)-endemic region (Otago, southern New Zealand); cardiac blood samples were taken on-site, and mononuclear cell cultures were established from 75 of these animals. Lymphocyte transformation (LT) responses to T cell and T/B cell mitogens (Concanavalin A Con A and pokeweed mitogen) were measured by uridine incorporation assay. The magnitude of these responses did not differ significantly between animals that had been captured in wire-framed cage traps and those captured using soft-jawed leg-hold traps. Serum cortisol and glucose (as indicators of physiological and oxidative stress, respectively) were highest in animals captured using leg-hold traps. In comparison to domesticated ferrets, wild-caught ferrets had lower overall LT responsesto Con A, but significantly higher levels of serum cortisol. 10 of 80 animals captured from the wild were severely diseased (Tb+), as evidenced by gross tuberculous lesions at PM examination. Mononuclear cell cultures were established from 9 of these animals; LT responses to Con A were significantly lower in Tb+ ferrets than in either wild caught/non-diseased (Tb-) or domesticated ferrets. These results show that it is possible to measure cellular immune responses from the blood of wild-caught ferrets,but that field capture and disease status may interfere with in vitro T cell function, possibly due to the effect of physiological stress.

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