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首页> 外文期刊>Journal of experimental & clinical cancer research >Immunohistochemical assessment of basement membrane components in colorectal cancer: prognostic implications.
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Immunohistochemical assessment of basement membrane components in colorectal cancer: prognostic implications.

机译：结直肠癌基底膜成分的免疫组织化学评估：预后意义。

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Loss of basement membrane integrity during neoplastic invasion may have some direct prognostic significance, which is worth investigating. We studied 151 cases of colorectal adenocarcinomas retrospectively. The aim of the study was to investigate the immunohistochemical expression as well as the distribution of laminin and collagen IV within the basement membranes of cancer cell formations. The results were related to histological grade of malignancy (I, II or III) and Dukes' staging of all tumours as well as to 3-year survival status in 52 patients. Using the immunostaining method of strept ABComplex/HRP and appropriate monoclonal or polyclonal antibodies, we assessed the continuity, the discontinuity of the distribution or the total loss of structural basement membrane components alongside the infiltrating borders of each tumour. The results were evaluated statistically. Either a considerable degree of discontinuity or a total loss of basement membrane components was more common in moderately and poorly differentiated adenocarcinomas (p = 0.002 and p = 0.005 for collagen IV and laminin, respectively) and they seemed to be adversely associated with survival status (p = 0.066 and p = 0.014 for collagen IV and laminin, respectively). Interestingly, no association with the stage of disease was noticed. The results of this study reinforce the value of laminin and collagen IV as possible prognostic factors independently to tumour stage. The total loss or considerable discontinuity of the basement membranes of cancerous cells can be considered as indicators of tumour aggressiveness.
机译：肿瘤浸润期间基底膜完整性的丧失可能具有一定的直接预后意义，值得研究。我们回顾性研究了 151 例结直肠腺癌病例。该研究的目的是研究免疫组织化学表达以及层粘连蛋白和胶原蛋白 IV 在癌细胞形成的基底膜内的分布。结果与恶性肿瘤的组织学分级（I、II 或 III）和 Dukes 对所有肿瘤的分期以及 52 例患者的 3 年生存状态有关。使用链球菌 ABComplex/HRP 和适当的单克隆或多克隆抗体的免疫染色方法，我们评估了结构基底膜成分的连续性、分布的不连续性或结构基底膜成分以及每个肿瘤浸润边界的完全丢失。对结果进行统计评估。在中度和低分化腺癌中，相当程度的不连续性或基底膜成分的完全丧失更常见（胶原 IV 和层粘连蛋白分别为 p = 0.002 和 p = 0.005），并且它们似乎与生存状态呈负相关（胶原 IV 和层粘连蛋白分别为 p = 0.066 和 p = 0.014）。有趣的是，没有发现与疾病阶段的关联。本研究的结果强化了层粘连蛋白和胶原蛋白 IV 作为独立于肿瘤分期的可能预后因素的价值。癌细胞基底膜的完全丧失或相当大的不连续性可以被认为是肿瘤侵袭性的指标。

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