Self-assembly of the general membrane-remodeling protein PVAP into sevenfold virus-associated pyramids

Bertram Daum; Tessa E. F. Quax; Martin SachseDeryck J. MillsJulia Reimann?zkan YildizSabine H?derCosmin SaveanuPatrick ForterreSonja-Verena AlbersWerner KühlbrandtDavid Prangishvili

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Self-assembly of the general membrane-remodeling protein PVAP into sevenfold virus-associated pyramids

机译：Self-assembly of the general membrane-remodeling protein PVAP into sevenfold virus-associated pyramids

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Viruses have developed a wide range of strategies to escape from the host cells in which they replicate. For egress some archaeal viruses use a pyramidal structure with sevenfold rotational symmetry. Virus-associated pyramids (VAPs) assemble in the host cell membrane from the virus-encoded protein PVAP and open at the end of the infection cycle. We characterize this unusual supramolecular assembly using a combination of genetic, biochemical, and electron microscopic techniques. By whole-cell electron cryotomography, we monitored morphological changes in virus-infected host cells. Subtomogram averaging reveals the VAP structure. By heterologous expression of PVAP in cells from all three domains of life, we demonstrate that the protein integrates indiscriminately into virtually any biological membrane, where it forms sevenfold pyramids. We identify the protein domains essential for VAP formation in PVAP truncation mutants by their ability to remodel the cell membrane. Self-assembly of PVAP into pyramids requires at least two different, in-plane and out-of-plane, protein interactions. Our findings allow us to propose a model describing how PVAP arranges to form sevenfold pyramids and suggest how this small, robust protein may be used as a general membrane-remodeling system.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2014年第10期|3829-3834|共6页
作者
Bertram Daum; Tessa E. F. Quax; Martin SachseDeryck J. MillsJulia Reimann?zkan YildizSabine H?derCosmin SaveanuPatrick ForterreSonja-Verena AlbersWerner KühlbrandtDavid Prangishvili;
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作者单位

Department of Structural Biology, Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany;

Biologie Moléculaire du Gène chez les Extrêmophiles, Institut Pasteur, 75015 Paris, France;

Plate-Forme de Microscopie Ultrastructurale, Institut Pasteur, 75015 Paris, FranceMolecular Biology of Archaea, Max-Planck Institute for Terrestrial Microbiology, 35043 Marburg, GermanyGénétique des Interactions Macromoléculaires, Institut Pasteur, 75015 Paris, France;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
archaea; archeovirus; viral egress;

Self-assembly of the general membrane-remodeling protein PVAP into sevenfold virus-associated pyramids

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