The enrichment of lipase-producing bacteria, isolated from the environment, was evaluated using a continuous cultivation system. Continuous cultivations were performed using a synthetic medium where the carbon and energy source, triolein, was provided physically entrapped in an external loop. Using this system, pure cultivations ofPseudomonas fluorescens378 showed an increase in lipase-producing ability, instead of declining as in more conventional systems. Enrichment cultures were obtained with environmental samples originating from a vegetable oil processing plant using the same system. Three types of lipase-producing bacteria were identified:P. alcaligenes, Enterobacter intermediumand a Gram-negative, oxidase-positive rod.
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