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首页> 外文期刊>european journal of phycology >Nitrate availability and calcite production inEmiliania huxleyiLohmann
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Nitrate availability and calcite production inEmiliania huxleyiLohmann

机译：Emiliania huxleyiLohmann 的硝酸盐可用性和方解石产量

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High-calcifying cells ofEmiliania huxleyiwere grown on a synthetic seawater medium and the effect of nitrate (NO-3) concentration on growth, calcite accumulation, calcification rate and DIC (dissolved inorganic carbon) utilisation determined. The stoichiometry between NO-3utilisation and calcite production was 1:6#xB7;5 (mol/mol). Calcification and growth were tightly coupled: calcite production ceased when cultures entered the stationary phase due to NO-3depletion, but by adding a pulse of NO-3growth and calcification were restored. The initial C/N ratio in the medium was important in relation to calcification rate. At 20 #xB5;M NO-3the total DIC (2 mM) was rapidly depleted, the calcification rate subsequently declining, whereas at 5 and 10 #xB5;M NO-3rates of calcification were constant at 20 g carbon cell-1#xD7; 1014#xB7;h-1throughout culture growth, excess DIC being present relative to the available NO-3. Calcite production per unit NO-3was similar for isolates ofE. huxleyifrom neritic, oligotrophic and nitrate-rich waters. In laboratory cultures, where the photon flux density is optimised for growth, the initial NO-3concentration is a reliable indicator of final calcite yield.
机译：在合成海水培养基上生长高钙化细胞，测定硝酸盐（NO-3）浓度对生长、方解石积累、钙化速率和DIC（溶解无机碳）利用的影响。NO-3利用与方解石生产之间的化学计量为1：6·5（mol/mol）。钙化和生长紧密耦合：当培养物由于 NO-3 消耗而进入固定期时，方解石的产生停止，但通过添加 NO-3 脉冲，生长和钙化得以恢复。培养基中的初始 C/N 比与钙化速率有关。在 20 μM NO-3 时，总 DIC （2 mM） 迅速耗尽，钙化速率随后下降，而在 5 μM 和 10 μM NO-3 时，钙化速率在 20 g 碳细胞-1 时恒定×在整个培养物生长过程中为 1014·h-1，相对于可用的 NO-3 存在过量的 DIC。单位NO-3的方解石产量与E分离株相似。赫胥黎来自富含硝酸盐的神经质、寡营养和硝酸盐的水域。在实验室培养中，光子通量密度针对生长进行了优化，初始 NO-3 浓度是最终方解石产率的可靠指标。

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