AbstractA method for measuring the concentration of recombinant retrovirus encoding theEscherichia coli LacZgene is described. The assay is based on the quantitative measurement of β‐galactosidase activity in extracts of cells infected with theLacZ‐encoding retrovirus.LacZ‐encoded β‐galactosidase activity in transduced cells is measured using the colorimetric substrate,O;‐nitrophenyl β‐D‐galactopyranoside (ONPG), and the results are read using an ELISA plate‐reader. Because the entire assay is performed in a 96‐well plate, large numbers of samples are easily measured, and the assay has the advantages of rapidity, precision, and ease of data collection. The assay was used to determine the optimal concentration of polybrene, a polycation known to enhance the infectivity of retroviruses, and can be used to evaluate other factors that affect infection, as well as the optimal conditions for production of the re
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