SummaryTheSaccharomyces cerevisiaeallergens were characterized by IgE‐immunoblotting with serum samples of 83 patients; 63 represented patients with atopic dermatitis with previous positive skin prick test or RAST forS. cerevisiae, seven patients with AD but negative test results and 13 were non‐atopic controls. Disrupted whole body extract ofS. cerevisiaewas used in the assays. From the patients tested 41 patients with atopic dermatitis appeared positive in IgE immunoblotting revealing 22 IgE stained bands. From these bands 10 represented intermediate allergens, and 12 minor allergens. The most frequent staining was obtained with the 48 kD band (39). When the staining pattern of 45 kD and 48 kD bands and mannan was compared withCandida albicansallergens or purified baker's yeast enolase a simultaneous binding was seen with the 48 kD band ofS. cerevisiaeand the 46 kD band ofC. albicansand enolase whereas the 45 kD band was neither associated with the 46 kD band ofC. albicansnor purified enolase. High molecular weight staining was found in five samples. The staining pattern was associated with the mannose containing structures in parallel withC. albic
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