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首页> 外文期刊>Journal of Experimental Botany >Fluxomics links cellular functional analyses to whole-plant phenotyping
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Fluxomics links cellular functional analyses to whole-plant phenotyping

机译：Fluxomics将细胞功能分析与全植物表型分析联系起来

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摘要

Fluxes through metabolic pathways reflect the integration of genetic and metabolic regulations. While it is attractive to measure all the mRNAs (transcriptome), all the proteins (proteome), and a large number of the metabolites (metabolome) in a given cellular system, linking and integrating this information remains difficult. Measurement of metabolome-wide fluxes (termed the fluxome) provides an integrated functional output of the cell machinery and a better tool to link functional analyses to plant phenotyping. This review presents and discusses sets of methodologies that have been developed to measure the fluxome. First, the principles of metabolic flux analysis (MFA), its ` short time interval' version Inst-MFA, and of constraints-based methods, such as flux balance analysis and kinetic analysis, are briefly described. The use of these powerful methods for flux characterization at the cellular scale up to the organ (fruits, seeds) and whole-plant level is illustrated. The added value given by fluxomics methods for unravelling how the abiotic environment affects flux, the process, and key metabolic steps are also described. Challenges associated with the development of fluxomics and its integration with ` omics' for thorough plant and organ functional phenotyping are discussed. Taken together, these will ultimately provide crucial clues for identifying appropriate target plant phenotypes for breeding.
机译：通过代谢途径的通量反映了遗传和代谢调控的整合。虽然测量给定细胞系统中的所有 mRNA（转录组）、所有蛋白质（蛋白质组）和大量代谢物（代谢组）很有吸引力，但链接和整合这些信息仍然很困难。代谢组通量的测量（称为通量组）提供了细胞机制的集成功能输出，以及将功能分析与植物表型联系起来的更好工具。本综述介绍并讨论了为测量通量组而开发的一系列方法。首先，简要介绍了代谢通量分析（MFA）的原理，其“短时间间隔”版本Inst-MFA，以及基于约束的方法，如通量平衡分析和动力学分析。说明了使用这些强大的方法在细胞尺度上到器官（果实、种子）和全植物水平上进行通量表征。还描述了通量组学方法在揭示非生物环境如何影响通量、过程和关键代谢步骤方面提供的附加值。讨论了与通量组学的发展及其与“组学”的整合相关的挑战，以彻底进行植物和器官功能表型分析。综上所述，这些最终将为确定合适的目标植物表型进行育种提供关键线索。

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