The potential of Streptococcus mutans C67–1, Bacterionema matruchotii ATCC 14266, Bacterionema sp. Ny 205, Rothia sp. Ny 21 and Neisseria sp. Ny 204 to form plaque and initiate caries was tested in mono-associated Osborne-Mendel rats and in di-associated rats in combination with Veillonella alcalescens OMZ 193. The proportions of the strains were assessed in pooled plaque samples. A decrease in the proportion of V. alcalescens was observed during the experimental period. A reduction of dentinal fissure lesions was found in the rats di-associated with V. alcalescens and Strep, mutans. Electron micrographs of the fissures revealed that V. alcalescens was not established in the fissures of rats di-associated with this strain and Bacterionema sp., Rothia sp. or Neisseria sp.; in these di-associated rats no reduction of the caries activity was observe
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