Identification and characterization of a novel promoter for the human ANO1 gene regulated by the transcription factor signal transducer and activator of transcription 6 (STAT6)

Mazzone Amelia; Gibbons Simon J.; Bernard Cheryl E.Nowsheen SomairaMiddha SumitAlmada Luciana L.Ordog TamasKendrick Michael L.Lombardo KMarie ReidShen K. RobertGalietta Luis J. V.Fernandez-Zapico Martin E.Farrugia Gianrico

首页> 外文期刊>The FASEB Journal >Identification and characterization of a novel promoter for the human ANO1 gene regulated by the transcription factor signal transducer and activator of transcription 6 (STAT6)

【24h】

Identification and characterization of a novel promoter for the human ANO1 gene regulated by the transcription factor signal transducer and activator of transcription 6 (STAT6)

机译：Identification and characterization of a novel promoter for the human ANO1 gene regulated by the transcription factor signal transducer and activator of transcription 6 (STAT6)

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Anoctamin-1 (Ano1) is a widely expressed protein responsible for endogenous Ca2+-activated Cl- currents. Ano1 is overexpressed in cancer. Differential expression of transcriptional variants is also found in other diseases. However, the mechanisms underlying regulation of Ano1 are unknown. This study identifies the Ano1 promoter and defines a mechanism for regulating its expression. Next-generation RNA sequencing (RNA-seq) analysis in human gastric muscle found a new exon upstream of the reported exon 1 and identified a promoter proximal to this new exon. Reporter assays in human embryonic kidney 293 cells showed a 6.7 +/- 2.1 fold increase in activity over empty vector. Treatment with a known regulator of Ano1 expression, IL-4, increased promoter activity by 1.6 +/- 0.02-fold over untreated cells. The promoter region contained putative binding sites for multiple transcription factors including signal transducer and activator of transcription 6 (STAT6), a downstream effector of IL-4. Chromatin immuno-precipitation (ChIP) experiments on T84 cells, which endogenously express Ano1, showed a 2.1 +/- 0.12-fold increase in binding of STAT6 to P0 after IL-4 treatment. These results were confirmed by mutagenesis, expression, and RNA interference techniques. This work allows deeper understanding of the regulation of Ano1 in physiology and as a potential therapeutic target in a variety of diseases

著录项

来源
《The FASEB Journal》 |2015年第1期|152-163|共12页
作者
Mazzone Amelia; Gibbons Simon J.; Bernard Cheryl E.Nowsheen SomairaMiddha SumitAlmada Luciana L.Ordog TamasKendrick Michael L.Lombardo KMarie ReidShen K. RobertGalietta Luis J. V.Fernandez-Zapico Martin E.Farrugia Gianrico;
展开▼
作者单位

Mayo Clin, Enter NeuroSci Program, Rochester, MN 55905 USA;

Mayo Clin, Mayo Med Sch, Rochester, MN 55905 USA;

Mayo Clin, Rochester, MN 55905 USAMayo Clin, Dept Oncol, Schulze Ctr Novel Therapeut, Rochester, MN 55905 USAMayo Clin, Dept Surg, Rochester, MN 55905 USAIst Giannina Gaslini, UOC Genet Med, I-16148 Genoa, Italy;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类生物化学;
关键词
TMEM16A; calcium-activated chloride channels; alternative splicing;

Identification and characterization of a novel promoter for the human ANO1 gene regulated by the transcription factor signal transducer and activator of transcription 6 (STAT6)

摘要

著录项

相关主题

期刊订阅