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首页> 外文期刊>Journal of Experimental Botany >Generation and characterization of a collection of knock-down lines for the chloroplast Clp protease complex in tobacco
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Generation and characterization of a collection of knock-down lines for the chloroplast Clp protease complex in tobacco

机译：烟草中叶绿体Clp蛋白酶复合物敲低系集合的生成和表征

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Protein degradation in chloroplasts is carried out by a set of proteases that eliminate misfolded, damaged, or superfluous proteins. The ATP-dependent caseinolytic protease (Clp) is the most complex protease in plastids and has been implicated mainly in stromal protein degradation. In contrast, FtsH, a thylakoid membrane-associated metalloprotease, is believed to participate mainly in the degradation of thylakoidal proteins. To determine the role of specific Clp and FtsH subunits in plant growth and development, RNAi lines targeting at least one subunit of each Clp ring and FtsH were generated in tobacco. In addition, mutation of the translation initiation codon was employed to down-regulate expression of the plastid-encoded ClpP1 subunit. These protease lines cover a broad range of reductions at the transcript and protein levels of the targeted genes. A wide spectrum of phenotypes was obtained, including pigment deficiency, alterations in leaf development, leaf variegations, and impaired photosynthesis. When knock-down lines for the different protease subunits were compared, both common and specific phenotypes were observed, suggesting distinct functions of at least some subunits. Our work provides a well-characterized collection of knockdown lines for plastid proteases in tobacco and reveals the importance of the Clp protease in physiology and plant development.
机译：叶绿体中的蛋白质降解是通过一组蛋白酶进行的，这些蛋白酶可以消除错误折叠、受损或多余的蛋白质。ATP 依赖性酪蛋白酶 （Clp） 是质体中最复杂的蛋白酶，主要与基质蛋白降解有关。相比之下，FtsH（一种类囊体膜相关的金属蛋白酶）被认为主要参与类囊体蛋白的降解。为了确定特定Clp和FtsH亚基在植物生长发育中的作用，在烟草中生成了靶向每个Clp环和FtsH的至少一个亚基的RNAi系。此外，翻译起始密码子的突变用于下调质体编码的 ClpP1 亚基的表达。这些蛋白酶系涵盖了靶基因转录本和蛋白质水平的广泛还原。获得了广泛的表型，包括色素缺乏、叶片发育改变、叶片杂色和光合作用受损。当比较不同蛋白酶亚基的敲低系时，观察到共同和特异性表型，表明至少某些亚基具有不同的功能。我们的工作为烟草中的质体蛋白酶提供了一组特征明确的敲低系，并揭示了Clp蛋白酶在生理学和植物发育中的重要性。

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