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外文期刊>Clinical and experimental allergy :
>Species‐specific measurement of the second group ofDermatophagoidesmite allergens, Der p 2 and Der f 2, using a monoclonal antibody‐based ELISA
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Species‐specific measurement of the second group ofDermatophagoidesmite allergens, Der p 2 and Der f 2, using a monoclonal antibody‐based ELISA
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机译：Species‐specific measurement of the second group ofDermatophagoidesmite allergens, Der p 2 and Der f 2, using a monoclonal antibody‐based ELISA
SummaryBackgroundThe group 2Dermatophagoidesmite allergens. Der p 2 and Der f 2, were known to he highly crossreactive, and previous assays to measure Der p 2 and Der f 2 were not species‐specific.ObjectiveThe aim of this study was to develop a monoclonal antibody‐based ELISA (MoAb‐ELISA) to specics‐spccifically measure Der p 2 and Der f 2.MethodsThe MoAb‐ELISA lor Der p 2 and Der f 2 was performed using species‐specific MoAbs for Der p 2 and Der f 2 and a biotinylated second MoAb which recognized a common epitope on both Der p 2 and Der f 2.RcsuitsThe assay was highly specics‐spccific, reproducible and sensitive. Thirty‐two house dust samples were assayed by the MoAb‐ELISA for Der p 2 and Der f 2 and by a previously reported radioimmunoassay for Der 2 with rabbit anti‐Der 2 antibodies. The summed values for Der p 2 and Der f 2 by the MoAb‐ELISA detnonstrated a good correlation with the Der 2 values using the radioimmunoassay (r = 0.978). Furthermore, the proportion of the Der p2level in the total Der 2 level (Der p 2 divided by Der p 2 plus Der f 2) correlated well with that of theD. pteronyssinusmite number to the totalDermalophagoidesmite number identificd by species (r = 0.970).ConclusionThe MoAb‐ELISA for Der p 2 and Der f 2, as well as that for Der p 1 and Der f 1, will be useful for the standardization of mite extracts and for the assessments of
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