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>Production of a copolyester of 3-hydroxybutyric acid and 3-hydroxyvaleric acid from single unrelated carbon sources by a mutant ofAlcaligenes eutrophus
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Production of a copolyester of 3-hydroxybutyric acid and 3-hydroxyvaleric acid from single unrelated carbon sources by a mutant ofAlcaligenes eutrophus
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机译：Production of a copolyester of 3-hydroxybutyric acid and 3-hydroxyvaleric acid from single unrelated carbon sources by a mutant ofAlcaligenes eutrophus
Alcaligenes eutrophusmutant strain R3, which is a spontaneous revertant to prototrophy of an isoleucine-auxotrophic mutant of the wild-type strain H16, accumulated a copolyester consisting of 3-hydroxybutyric acid (3HB) as main constituent and of 3-hydroxyvaleric acid (3HV), i.e. poly(3HB-co-3HV), as the only other constituent from various single unrelated substrates, which were provided in excess, after a nutrient essential for growth was depleted in the medium. Poly(SHB-co-3HV) was produced from fructose, gluconate, succinate, acetate or lactate during cell starvation of the nitrogen, sulphur or magnesium source. Although 3HV amounted to only 8 mol of the constituents of the polyester, this study provides a general rationale for construction and utilization of mutants of poly(3HB)-accumulating bacteria that are altered in the metabolism of branched-chain amino acids for the production of poly(3HB-co-3HV) from single unrelated substrates.
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