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外文期刊>Clinical and experimental allergy :
>Use of an autologous reactionin vitroto assess contributions of T and B lymphocytes to immune hyperreactivity of atopics
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Use of an autologous reactionin vitroto assess contributions of T and B lymphocytes to immune hyperreactivity of atopics
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机译：Use of an autologous reactionin vitroto assess contributions of T and B lymphocytes to immune hyperreactivity of atopics
SummaryThein‐vitroproliferative reaction of peripheral blood lymphocytes (measured by 3Hthymidine incorporation) to autologous pokeweed mitogen (PWM)‐induced lymphoblasts (PWM‐lymphoblast‐stimulated autologous mixed leucocyte reaction, PWM.AMLR) was used as a measure of immune hyperreactivity for comparison of atopic with non‐atopic individuals. Accordingly, 10/24 non‐atopics responded in the PWM.AMLR, and 19/19 atopics reacting to inhaled allergens responded. Autologous stimulation was associated with release of mitogenic factors from the PWM‐activated stimulating cells (2/15 non‐atopics, 9/15 atopics). For non‐atopics, stimulation delivered by staphylococcus A (SAC)‐activated cells was similar to that delivered by PWM‐induced cells, while in atopics, the SAC.AMLR was never more than 50 of the PWM.AMLR, indicating a possible T cell component. Separation by panning of the stimulation cells into lymphocyte subsets supported the notion that stimulation involved a cooperation between B and T4+T cells. It is proposed that a positive PWM.AMLR is dependent upon an initial B cell activation followed by the PWM stimulus dependent upon a previous T cell activation, where atopics have more lymphocytes in an activated state than healthy non‐atopics. Such a baseline priming may contribute to an innate sensitivity of atopics to
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