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Influence of the Distribution of the Manganese-Oxidizing Bacterium, Leptothrix Discophora, on Ennoblement of Type 316L Stainless Steel

机译:Influence of the Distribution of the Manganese-Oxidizing Bacterium, Leptothrix Discophora, on Ennoblement of Type 316L Stainless Steel

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摘要

Type 316L (UNS 31603) stairdess steel (SS) was ennobled to an open-circuit potential (OCP) of 323 mV vs saturated calomel electrode (SCE) within a 5-day period due to the deposition of manganese oxides on the metal surface by a monospecies biofilm of Leptothrix discophora. However, the same metal experienced only partial ennoblement, achieving a maximum potential of 143 mV_(SCE) during the same period when colonized by a three-species biofilm containing L. discophora, and even this level of ennoblement by the mixed-species biofilm was only transient since the potential decreased to 122 mV_(SCE) shortly after achieving the maximum potential. The mixed-species bio?lm was significantly thicker (>200 mu m) than the L. discophora monospecies biofilm (120 mu m). Using 16s rRNA probes specific forL. discophora, fluorescent in-situ hybridization revealed cells to be heteroge-neously distributed throughout the monospecies biofilm. In the three-species bio?lm, 16_s rRNA probes revealed a homo-geneous layer of L. discophora that resided proximal to the biofilm-bulk solution phase. At the most distal position from the bio?lm-bulk solution phase near the glass-biofilm interface there existed very few to noL. discophora. Microelec-trode studies revealed the presence of oxygen (3.85 mg/L to 4.35 mg/L) at the monospecies-glass substratum interface in 50 percent of the areas of the substratum assayed. No oxygen was detected at the three-species biofilm-glass interface where the thickness of the overlying bio?lm exceeded 200 mu m. The results suggest that ennoblement of Type 316L SS by L. discophora depends on oxygen accessibility to cells proximal to the substratum.

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