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>Occupational allergy in an entomological research center. II. Identification of IgE‐binding proteins from developmental stages of the blowflyLucilia cuprinaand other species of adult flies
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Occupational allergy in an entomological research center. II. Identification of IgE‐binding proteins from developmental stages of the blowflyLucilia cuprinaand other species of adult flies
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机译：Occupational allergy in an entomological research center. II. Identification of IgE‐binding proteins from developmental stages of the blowflyLucilia cuprinaand other species of adult flies
SummarySera from 30 workers in an entomological research institute and from five Sydney asthmatics, all with serum IgE antibodies to sheep blowfly (Lucilia cuprina) antigens, were studied with the aim of identifying individual IgE‐binding proteins in extracts ofL. cuprinaadult flies, larvae and eggs, and in extracts of related species of flies from the order Diptera. Using protein blotting, 21, 18 and nine different IgE‐binding components were identified in extracts ofL. cuprinaadult flies, larvae and eggs, respectively. A component(s)of MW 67 kD found in all three developmental stages, showed the highest frequency of IgE‐binding; reacting, for example, with 70 of the sera tested with the adult fly extract. Some components were detected in only one of the three developmental stages. Investigations of possible allergenic cross‐reactivity betweenL. cuprinaand extracts from six other related species of flies revealed IgE‐binding bands in each of the extracts as well as in an extract ofAgrotis infusa, a cutworm not belonging to the order Diptera. One strongly reacting component of MW 20 kD was detected in extracts of five different fly species as well as in theA. infusaextract. The results suggested that allergenic cross‐reactivity between some fly species exists, and may extend to taxonomically unrelated ins
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