AbstractThe genomic organization of four oncogenes,c‐myc,c‐myb,c‐Ha‐ras, andv‐fms, was analyzed in 21 patients with malignant bone tumors. Amplification of thec‐mycproto‐oncogene without rearrangement was the sole abnormality detected in four tumors: two chondrosarcomas, one osteosarcoma, and one lymphoma of bone. DNA hybridizations withc‐myb,c‐Ha‐ras, andv‐fmsprobes disclosed no structural gene abnormalities. Point mutations at the 12th codon of thec‐Ha‐rasgene were investigated with the polymerase chain reaction technique; no alterations were detected. The observed amplification of thec‐mycthere was not related to histologic type, grade, surgical stage, or ploidy level of the tumors. The results indicated thatc‐mycamplification, presumed to be involved in the development of malignancy in a variety of solid tumors, is encountered sporadically in malignant bone tumors; however, this occurs without relation to common histopathologic features. The clinical significance of oncogene amplification in bone sarc
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