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>Photosynthesis, growth and hydrocarbon production ofBotryococcus brauniiimmobilized by entrapment and adsorption in polyurethane foams
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Photosynthesis, growth and hydrocarbon production ofBotryococcus brauniiimmobilized by entrapment and adsorption in polyurethane foams
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机译:Photosynthesis, growth and hydrocarbon production ofBotryococcus brauniiimmobilized by entrapment and adsorption in polyurethane foams
Direct entrapment of the hydrocarbonrich algaBotryococcus brauniiwas examined using eleven polyurethane prepolymers. A high toxicity was observed in several foams. With five of the tested prepolymers, nevertheless, a large part of the algal population can survive entrapment and substantial photosynthetic capacity, ca. 40–60 relative to free controls, was retained one day after immobilization. However, prolonged batches under standard conditions revealed a long-term toxicity; as a result the photosynthetic capacity and hydrocarbon production of the entrapped cultures were strongly reduced relative to free controls. Immobilization ofB. brauniiwas also achieved, with a loading yield of ca. 70, via adsorption on FHP 4000 and FHP 5000 foams. Subsequent batch cultures under shaken and airlift conditions revealed a substantial release, ca. 30 of free cells, at the end of the cultures. However, the release from these adsorbed cultures was no higher than from directly entrappedB. braunii. Furthermore, no toxic effects were noted in the adsorbed cultures; the showed active growth, high photosynthetic capacity and produced quite large amounts of hydrocarbons, the chemical structure and the relative abundance of which were not altered by immobilization. Taking into account cell leakage, it appears that adsorbed cultures exhibit a similar, and sometimes even higher metabolic activity than free controls; thus, under air-lift conditions, high biomass and hydrocarbon productivities can be achieve
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