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首页> 外文期刊>Food science and biotechnology >Isolation and identification of azelaic acid and 3,4-dihydroxybenzoic acid from buckwheat hull as antimicrobial substances
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Isolation and identification of azelaic acid and 3,4-dihydroxybenzoic acid from buckwheat hull as antimicrobial substances

机译:Isolation and identification of azelaic acid and 3,4-dihydroxybenzoic acid from buckwheat hull as antimicrobial substances

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摘要

The methanol extract of buckwheat hull showed antimicrobial activity against bacteria. The extract was successively purified through solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 column chromatography, and octadecylsilane column chromatography. Further purification through high performance liquid chromatography gave two antimicrobial substances which were, respectively, identified as azelaic acid and 3,4-dihydroxybenzoic acid through MS and NMR analyses. The compounds showed antimicrobial activity against gram-positive and gram-positive and gram-negative bacteria at 200-500 #mu#g of concentration using the paper disc method.

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