...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The FASEB Journal >Reduced prostaglandin I-2 signaling in Arid5b(-/-) primary skeletal muscle cells attenuates myogenesis
【24h】

Reduced prostaglandin I-2 signaling in Arid5b(-/-) primary skeletal muscle cells attenuates myogenesis

机译：Reduced prostaglandin I-2 signaling in Arid5b(-/-) primary skeletal muscle cells attenuates myogenesis

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

The AT-rich interaction domain (ARID) family of proteins regulates gene expression, development, and differentiation. Although Arid5b has important functions in adipogenesis and chondrogenesis, the role of Arid5b in skeletal muscle myogenesis has not been investigated. Therefore, we isolated primary skeletal muscle cells from Arid5b(+/+) and Arid5b(-/-) mice and characterized differentiation in these cells. We found that Arid5b(-/-) primary skeletal muscle cells showed differentiation defects and impaired sarcomeric assembly. Microarray analysis revealed down-regulation of the prostanoid biosynthesis pathway in Arid5b(-/-) myoblasts, including the genes encoding cyclooxygenase (COX)-1 (Ptgs1) and prostaglandin (PG) I synthase (Ptgis). Down-regulation of COX-1 and PGI synthase was confirmed by real-time PCR and Western blot analyses. Correspondingly, the production of PGI(2), as measured by ELISA, was reduced in Arid5b(-/-) cells relative to Arid5b(+/+) cells. Boyden chamber assays showed that migration was increased but chemotaxis was impaired in Arid5b(-/-) cells. Myoblast fusion was also inhibited in Arid5b(-/-) cells compared with Arid5b(+/+) cells. Treatment with the PGI(2) analog iloprost rescued the defects in myotube formation, migration, and fusion. These results demonstrate that Arid5b has a novel and essential role in skeletal muscle differentiation by regulating PGI(2) production.

著录项

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号