Capillary electrophoresis equipped with chemiluminescence detector using 1,10-phenanthroline reagent was newly developed for analysis of metal ions. Cu(II), Co(II), or Fe(III) solution as a sample was introduced into the capillary and migrated with a Trisboric acid buffer (pH 9.0). They were all detected at ca. 5.2 min, and determined over the range of 5 × 10{sup}(-9) - 5 × 10{sup}(-5), 5 × 10{sup}(-7) - 1 × 10{sup}(-4), and 5 × 10{sup}(-7) - 1 × 10{sup}(-4) M, respectively. The present method was about 10-1000 times as sensitive as those using an absorption detector. When using an imidazole-HCl buffer (pH 7.0), only Cu(II) sample appeared clearly with two peaks on the electropherogram. This interesting behavior may be based on the coordination of imidazole molecule to Cu(II).
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