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首页> 外文期刊>Cell motility and the cytoskeleton >β‐actin specific monoclonal antibody
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β‐actin specific monoclonal antibody

机译：β‐actin specific monoclonal antibody

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AbstractUsing a synthetic peptide mimicking the NH2‐terminus of β‐actin we have raised a monoclonal antibody specific for this cytoplasmic actin isoform. Specificity of the antibody was demonstrated by its labelling of the actin polypeptide only in tissues containing the β isoform, by its exclusive recognition of the synthetic β‐actin peptide amongst those mimicking all six vertebrate isoactins, and by its selective recognition of the β‐actin spot in two‐dimensional electrophoresis gels of smooth muscle extracts. The antibody bound to actin filaments in both living and fixed fibroblasts where it labelled the stress fiber bundles and, more predominantly, the peripheral actin rich lamellipodia. The characteristics of the antibody indicate that it should serve as a useful tool for studying isoactin distribution and function. © 1994 W

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