首页>
外文期刊>journal of cellular physiology
>Differentiation of human keratinocytes: Changes in lipid synthesis, plasma membrane lipid composition, and125I‐EGF binding upon administration of 25‐hydroxycholesterol and mevinolin
【24h】
Differentiation of human keratinocytes: Changes in lipid synthesis, plasma membrane lipid composition, and125I‐EGF binding upon administration of 25‐hydroxycholesterol and mevinolin
展开▼
机译：Differentiation of human keratinocytes: Changes in lipid synthesis, plasma membrane lipid composition, and125I‐EGF binding upon administration of 25‐hydroxycholesterol and mevinolin
AbstractWe have studied the relationship between differentiation capacity, plasma membrane composition, and epidermal growth factor (EGF) receptor expression of normal keratinocytes in vitro. The plasma membrane composition of the cells was modulated experimentally by cholesterol depletion, using specific inhibitors of cholesterol synthesis, such as 25‐hydroxycholesterol and mevinolin. Exposure of the cells towards these inhibitors resulted in a drastic decrease of cholesterol biosynthesis, as determined from14C‐acetate incorporation into the various lipid fractions. This effect on cholesterol biosynthesis was reflected by changes in plasma membrane composition, as determined by lipid analysis of isolated plasma membrane fractions, these resulting in a decreased cholesterol‐phospholipid ratio. The experimental modulation of plasma membrane composition by 25‐hydroxycholesterol or mevinolin were accompanied by a decreased cornified envelope formation and by high expression of EGF binding sites. These phenomena were more pronounced in cells induced to differentiate by exposure of cells grown under low Ca2+to normal Ca2+concentrations, as compared to cells grown persistantly under low Ca2+concentrations. These results suggest a close correlation between plasma membrane composition, differentiation capacity, and EGF receptor exp
展开▼
