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>Characterization of intermediate filaments and their structural organization during epithelium formation in pigmented epithelial cells of the retina in vitro
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Characterization of intermediate filaments and their structural organization during epithelium formation in pigmented epithelial cells of the retina in vitro
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机译：Characterization of intermediate filaments and their structural organization during epithelium formation in pigmented epithelial cells of the retina in vitro
Retinal pigmented epithelial cells of chicken have circumferential microfilament bundles (CMBs) at the zonula adherens region. Isolated CMBs are polygons filled with a meshwork composed primarily of intermediate filaments; they show three major components of 200000, 55000, and 42000 daltons in SDS-gel electrophoresis. Here we have characterized the 55000-dalton protein immunochemically and ultrastructurally. Immunoblotting and immunofluorescence microscopy have shown that the 55000-dalton protein is an intermediate filament protein, vimentin.
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