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外文期刊>european journal of neuroscience
>Hydroxyindole‐O‐methyltransferase in the Chicken Retina: Immunocytochemical‐ Localization and Daily Rhythm of mRNA
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Hydroxyindole‐O‐methyltransferase in the Chicken Retina: Immunocytochemical‐ Localization and Daily Rhythm of mRNA
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机译：Hydroxyindole‐O‐methyltransferase in the Chicken Retina: Immunocytochemical‐ Localization and Daily Rhythm of mRNA
AbstractIn the vertebrate retina and pineal gland, melatonin production displays diurnal variations with high levels at night. Hydroxyindole‐O‐methyltransferase (HIOMT, EC 2.1.1.4) catalyses the last step of melatonin biosynthesis. In the present study, a cDNA encoding chicken HIOMT was used to examine the effects of environmental lighting on HIOMT mRNA expression in the chicken retina. A day/night rhythm of HIOMT mRNA level was observed, with an average 5‐fold increase during the night. Light strongly suppressed the night‐time rise in HIOMT mRNA concentration while darkness prevented its daytime fall. An antibody directed against chicken HIOMT was used for immunocytochemical identification of retinal melatoninergic cells. HIOMT immunoreactivity could be observed in rods as well as in cones. However, the lowest levels of HIOMT immunoreactivity were always observed in the accessory cones of double cones. A few HIOMT‐positive cell bodies could also be observed in the inner nuclear layer. Altogether, these data indicate that HIOMT gene expression in the retina is organized on a daily basis as a direct response to light, and that the different types of photoreceptors may not be equally involved in melatonin p
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