AbstractAt the terminal web of the chicken intestinal epithelial cell, the actin bundles are cross‐linked by a fine filamentous network of actin‐associated cross‐linkers. Myosin, fodrin, and TW 260/240 have been identified as major components of the cross‐linkers. We studied the development of the cross‐linkers by quick‐freeze, deep‐etch electron microscopy, and the expression of cross‐linker proteins (myosin, fodrin 240, and TW 260) by immunofluorescence and immunoblotting analysis during the embryogenesis. Microvilli start to form at 5–7 days, and the rootlets begin to elongate at 10 days. At an early stage of the development of the terminal web (13 days), fodrin 240 and a small amount of myosin are expressed, and a few actin‐associated cross‐linkers are present between the rootlets. However, TW 260 is not expressed at this stage. At an intermediate stage (19 days), the amount of myosin increases, and TW 260 begins to be expressed. The number of cross‐linkers associated with the unit length of the rootlets is 24/μm. At the final stage of the terminal web formation (2 days after hatching), the amount of fodrin 240, myosin, and TW 260 is similar to the adult level, and the number of the actin‐associated cross‐linkers per unit length of the rootlet is 27/μm (∼︁85 of the adult). These results suggest that the synthesis of cross‐linker proteins may be intricately regulated to achieve the desired density of cross‐linkages at each developmental stage: at early and intermediate stages, sufficient and not an excess of cross‐linkages are formed; and at a final stage, a higher complexity of cross‐linkages is achieved. In addition, there is a differential expression of the components of the actin‐associated cross‐linkers: myosin and fodrin could be early components of the cross‐linkers involved in the basic stabilization of the terminal web structure, whereas TW 260/240 becomes incorporated later, possibly involved in the stabilization preparatory to the rapid elongation of microvilli, which
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