The bioavailability of ingested methylmercury (CH{sub}3Hg(II)) was investigated in vitro using the gastric and intestinal fluids of channel catfish, Ictalurus punctatus, and Atlantic sturgeon, Acipenser oxyrinchus. Gastric fluid collected from each species was incubated with CH{sub}3Hg(II)-spiked sediment or bloodworms, after which the intestinal fluid of each species was added and incubated further. The proportion of CH{sub}3Hg(II) solubilized from bloodworms and sediment appeared to be controlled by complexation to amino acids in both the stomach and the intestinal fluids during the digestive process, with the more thorough digestion of bloodworm organic material enhancing CH{sub}3Hg(II) solubilization. A greater proportion of CH{sub}3Hg(II) was solubilized by the sturgeon fluids compared to the catfish fluids, especially for the sediment incubations. These differences corresponded to the relative amount of amino acids in the fluids of these fish. A comparison of the catfish gastrointestinal solubilization incubations and a CH{sub}3Hg(II) bioaccumulation experiment with bloodworms revealed that the solubilization incubations may be a reasonable surrogate measurement of the bioavailability of CH{sub}3Hg(II) to fish. Overall, it appears that digestive processes is the most important controlling factor in the bioavailability of CH{sub}3Hg(II) to fish.
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