• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and experimental allergy : >Effects of IL‐4 and IL‐13 on total and allergen specific IgE production by cultured PBMC from allergic patients determined with recombinant pollen allergens
【24h】

Effects of IL‐4 and IL‐13 on total and allergen specific IgE production by cultured PBMC from allergic patients determined with recombinant pollen allergens

机译：Effects of IL‐4 and IL‐13 on total and allergen specific IgE production by cultured PBMC from allergic patients determined with recombinant pollen allergens

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

SummaryBackground:Interleukin (IL)‐4 and IL‐13 have been shown to be potent switch factors for IgE synthesis in human B cells.Objective:In this study we investigated the effects of recombinant human IL‐4 and IL‐13 on total and allergen specific IgE synthesis by peripheral blood inononuclear colls (PBMC) from pollen allergic patients and healthy control individuals.Methods:Peripheral blood mononuclear cells (PBMC) from allergic patients were investigated for their capacity to produce allergen specific IgEin vitro. Total protein extracts from birch pollen and timothy grass pollen as well as purified recombinant birch pollen allergens,Betv I, birch profiling (Betv II) and recombinant timothy grass pollen allergens.Phi pI,Phi pII, andPhi pV were used to measure specific IgE‐antibody synthesis in cell culture supernatants by IgE‐immunoblot and ELISA.ReultsPBMC obtained from allergic patients spontaneously secreted allergen specific IgE in the culture supernatants. Addition of Interleukin 4, Interleukin 13 and anti‐CD40 antibody to the cultures alone or in combinations significantly induced total IgE production whereas allergen specific IgE production was not affected.Conclusion:Our results indicate that the peripheral blood of allergic individuals contains long lived allergen specific B cells which have already switched to IgE production and which are not sensitive to IL‐4 and IL‐13 treatment. These results may have implications on attempts to use cytokines or cytokine antagonists in therapy

著录项

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号