首页>
外文期刊>journal of cellular physiology
>Cell growth and differentiation in vitro in mouse macrophages transformed by atsAmutant of simian virus 40. III. Large T antigen level and cell proliferation and survival in an SV40tsA640‐transformed macrophage line
【24h】
Cell growth and differentiation in vitro in mouse macrophages transformed by atsAmutant of simian virus 40. III. Large T antigen level and cell proliferation and survival in an SV40tsA640‐transformed macrophage line
展开▼
机译：Cell growth and differentiation in vitro in mouse macrophages transformed by atsAmutant of simian virus 40. III. Large T antigen level and cell proliferation and survival in an SV40tsA640‐transformed macrophage line
AbstractThe levels of simian virus 40 (SV40) large T antigen in atsA‐transformed mouse macrophage line at the permissive (33°C) and the nonpermissive (30°C) temperature were examined by immunofluorescence, sodium dodecylsulfate‐polyacrylamide gel electrophoresis, complement fixation, and enzyme‐linked immunosorbent assay. When the cells were confluent and rested at 33°C, and then were shifted to 39°C, the amount of large T antigen per cell decreased, and most cells survived and remained phagocytic. When the cells were proliferating at 33°C, and then were shifted to 39°C, the cells died with only a small reduction in the amount of large T antigen. Therefore, the physiological state of the cells may determine the survival of cells by affecting the level of large T antigen after exposure to 39°C. The confluent cells may be rested with a concomitant decrease of large T antigen. The proliferating cells may not survive in the presence of a relatively high level of functionally defective large T ant
展开▼
