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外文期刊>european journal of neuroscience
>Characteristics of Calcium Channels Responsible for Voltage‐activated Calcium Entry in Rat Cerebellar Granule Cells
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Characteristics of Calcium Channels Responsible for Voltage‐activated Calcium Entry in Rat Cerebellar Granule Cells
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机译：Characteristics of Calcium Channels Responsible for Voltage‐activated Calcium Entry in Rat Cerebellar Granule Cells
AbstractThe properties and characteristics of calcium channel openings in cerebellar granule cells were analysed by the cell‐attached patch‐clamp technique. At depolarized potentials, with 110 mM Ba2+ as the divalent charge carrier, 36 of the patches displayed activity that consisted of elementary events whose amplitude ranged from – 0.3 to –1. 75 pA at 0 mV, giving rise to a high threshold current. In this population of events at least four different types of channel openings were identified by their distinct biophysical and pharmacological properties. Two types of channel openings, with conductances around 24 and 7 pS, had similar characteristics in that both opened following two modes of gating characterized by brief (˜ 2 ms) and longer openings (˜ 8 ms) and both were sensitive to dihydropyridines. A further type of channel opening, with a conductance around 11 pS gated mainly with brief openings (∼1 ms), was shown to be insensitive to dihydropyridines but was undetectable in recordings from the cells that had been treated with ω‐conotoxin. The last type of event was revealed after treatment of the cell with nicardipine or nifedipine and ω‐conotoxin. The corresponding channel had a conductance of 19 pS and opened in one dominant mode characterized by brief openings (∼1 ms). The data obtained on single‐channel activity of cerebellar granule cells are compared with the properties of the total current recorded in
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