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>A pharmacological and autoradiographic study on the ultrastructural localization of indoleamine synthesis in the rabbit pineal gland
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A pharmacological and autoradiographic study on the ultrastructural localization of indoleamine synthesis in the rabbit pineal gland
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机译：A pharmacological and autoradiographic study on the ultrastructural localization of indoleamine synthesis in the rabbit pineal gland
In order to localize pineal indoleamine synthesis at the ultrastructural level, an electron microscopic analysis was carried out on rabbit pineal tissue, cultured for several days in a medium containing the inhibitor p-chlorophenylalanine. Furthermore, electron microscopic autoradiography was applied to rabbit pineal tissue cultured in a medium containing the tritium labeled precursor 5-hydroxytryptophan. p-Chlorophenylalanine altered the ultrastructure of the mitochondria in the light pinealocytes only. Incubation with3H-5-hydroxytryptophan caused a moderate and uniform labeling of the pinealocytes. These data suggest that the synthesis and storage of indoleamines in the rabbit pineal gland do not take place within the conventional membrane-limited cell organelles but more diffusely in the cytosol of the light pinealocytes.
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