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>Basic fibroblast growth factor and insulin‐like growth factor I are strong mitogens for cultured mouse keratinocytes
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Basic fibroblast growth factor and insulin‐like growth factor I are strong mitogens for cultured mouse keratinocytes
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机译：Basic fibroblast growth factor and insulin‐like growth factor I are strong mitogens for cultured mouse keratinocytes
AbstractBasic fibroblast growth factor (bFGF), but not acidic fibroblast growth factor (aFGF), was found to be mitogenic for cultured mouse keratinocytes. A six‐to‐nine fold increase in3H‐thymidine (3H‐dT) incorporation into the acid insoluble pool and a similar increase of the labeling index can be measured when bFGF, at a concentration between 1 and 10 ng/ml, is added to keratinocytes arrested in serum‐free and growth factor‐free medium with a Ca++‐concentration below 0.1 mM. The half‐maximal response is observed between 0.2 and 0.7 ng/ml. In the same culture system, insulin‐like growth factor I/somatomedin C (IGF‐I) and insulin with 100–500 ng/ml. Basic FGF, IGF‐I and insulin can be classified as strong stimulators of DNA synthesis in mouse keratinocytes. In this regard they are comparable to epidermal growth factor, which shows a half‐maximal stimulation at a concentration between 1.5–2 ng/ml. These results show that in addition to mesenchymal cells, FGF is a growth factor not only for neuroectodermal cells, but ectodermal cells in general. They further support the idea that the growth promoting effect of insulin on keratinocytes may be med
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