...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>journal of cellular physiology >Down‐regulation of retinoic acid receptor activity associated with decreased α and γ isoforms expression in F9 embryonal carcinoma cells differentiated by retinoic acid
【24h】

Down‐regulation of retinoic acid receptor activity associated with decreased α and γ isoforms expression in F9 embryonal carcinoma cells differentiated by retinoic acid

机译：Down‐regulation of retinoic acid receptor activity associated with decreased α and γ isoforms expression in F9 embryonal carcinoma cells differentiated by retinoic acid

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

AbstractF9 embryonal carcinoma cells differentiate in response to retinoic acid (RA). To investigate the regulation of RA receptors (RARs) expression during this process, cDNA probes specific for the major RAR isoforms were used. In contrast to the level of RARβ2 mRNA which was high in cells treated 5 days with RA and below detection in untreated cells, as previously described, the steady state levels of RARα1, α2, γl, and γ2 mRNAs were markedly decreased in the RA‐differentiated cells as compared to untreated cells. The down‐regulation of the RA‐responsive system in differentiated cells was also evident in gel shift assays as a marked decrease in binding capacity to a retinoid acid response element (βRARE), as well as in chloramphenicol acetyltransferase (CAT) assays as a sixfold decrease in RA‐mediated transacting activity via this element. The down‐regulation of RAR DNA‐binding and transacting activity coincided with the burst in tissue plasminogen activator secretion and thus, occurred at the hinge between early and late differentiation. The down‐regulation of RA responsiveness may constitute an important event in the transition between early and late differentiation stage in F9 cells. ©

著录项

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号