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首页> 外文期刊>Assiut Veterinary Medical Journal >CLINICAL, PARASITOLOGICAL, SEROLOGICAL, AND MOLECULAR DIAGNOSIS OF TRYPANOSOMIASIS IN CAMELS (CAMELUS DROMEDARIUS)
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CLINICAL, PARASITOLOGICAL, SEROLOGICAL, AND MOLECULAR DIAGNOSIS OF TRYPANOSOMIASIS IN CAMELS (CAMELUS DROMEDARIUS)

机译：CLINICAL, PARASITOLOGICAL, SEROLOGICAL, AND MOLECULAR DIAGNOSIS OF TRYPANOSOMIASIS IN CAMELS (CAMELUS DROMEDARIUS)

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Trypanosomiasis the most prevalent disease of camels, this study was carried out 106 one-humped camel (Camelus dromedarius) in Aswan. indicated 18 camels suffered from clinical signs of Trypanosomiasis as weakness, rough coat, Pale mucous membrane and anemia, Emaciation and thinning of the hump and prominent ribs, fever, watery eye, and Diarrhea, and by Microscopic examination Trypanosoma showed in 7 stained blood film of camels (6.6), out from 106 serum samples were 13 (12.26) camels give positive results by Formol Gel Test including only one sample positive by blood film test. When using PCR test only 15 camels of them were tested and (13) 86.7 of them were found to be positive for the presence of TBR genome. When tested by Rhode Trypanozoon antigenic type (RoTat1.2) PCR targets all the 15 samples were negative that may be due to the Trypanosomes may be Trypanosoma evansi type B (lacks of RoTat genome) which may newly introduced to Egypt or may the sequence assorted variety of the RoTat1.2 gene in trypanosoma in Egypt detected from camels, especially in relationship with parasite long perseverance in camels because of the chronic period of the disease. Therefore, TBR-PCR was the more specific and sensitive method of the all methods used during this study. The suspected camel showed significant decreases in total erythrocytes count (TEC), hemoglobin (Hb), and a non significant increase in total leucocyte count (TLC), There was a significant increase in Alanine Amino Transferase (ALT), Total protein, globulin show a significant increase while glucose, Aspartate Amino Transferase (AST) level didn't differ significantly.

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