Distinction of the Skin Flukes Benedenia seriolae and Neobenedenia girellae Infecting Seriola spp. by PCR-RFLP Assay

Keigo Kobayshi; Germaine Lau Gek Khin; Sho Shirakashi

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Distinction of the Skin Flukes Benedenia seriolae and Neobenedenia girellae Infecting Seriola spp. by PCR-RFLP Assay

机译：Distinction of the Skin Flukes Benedenia seriolae and Neobenedenia girellae Infecting Seriola spp. by PCR-RFLP Assay

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We developed a PCR-RFLP method to distinguish Benedenia seriolae and Neobenedenia girellae, both infecting amberjacks Seriola spp. more precisely than a morphological method. PCR products of 28S rRNA and COX1 were digested by C/a I and EcoR I, respectively. For all individuals of B. seriolae, the digestions split each of the PCR products into two fragments. On the other hand, although the products of most of the N. girellae samples were digested by neither the enzymes, only PCR products of 28S rRNA gene of three of the individuals were split into three fragments, suggesting they were the hybrid between the two species.

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《魚病研究》 |2018年第4期|124-127|共4页
作者
Keigo Kobayshi; Germaine Lau Gek Khin; Sho Shirakashi;
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作者单位

Laboratory of Genome Science, Tokyo University of Marine Science and Technology,Tokyo 108-8477, Japan;

Aquaculture Research Institute, Kindai University, Wakayama 649-2211, Japan;

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原文格式 PDF
正文语种英语
中图分类水产保护学;
关键词
capsalid; monogenea; PCR-RFLP; Seriola spp.; skin fluke;

Distinction of the Skin Flukes Benedenia seriolae and Neobenedenia girellae Infecting Seriola spp. by PCR-RFLP Assay

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