AbstractA new biocomposite, fabricated by incorporating lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide (NAD+) within a graphite‐epoxy matrix, is used as a polishable (renewable) and robust biosensor for lactate. Highly sensitive and rapid amperometric biosensing is accomplished at relatively low potentials. The regeneration capability of the biocomposite probe circumvents problems of surface fouling inherent to the detection of NADH. Flow injection detection of lactate, with a sample throughput of 60/h, detection limit of 5 × 10−4M, precision of 2.5 (RSD forn= 40), and linearity up to 2 × 10−2M, is illu
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