Initial increase and subsequent loss of vasoactive intestinal polypeptide immunoreactivity in acetylcholinesterase-positive neurons of mouse islets transplanted to the kidney

SolveigPersson-Sjögren; StureForsgren; PålRoothInge-BertTäljedal

首页> 外文期刊>cell and tissue research >Initial increase and subsequent loss of vasoactive intestinal polypeptide immunoreactivity in acetylcholinesterase-positive neurons of mouse islets transplanted to the kidney

【24h】

Initial increase and subsequent loss of vasoactive intestinal polypeptide immunoreactivity in acetylcholinesterase-positive neurons of mouse islets transplanted to the kidney

机译：Initial increase and subsequent loss of vasoactive intestinal polypeptide immunoreactivity in acetylcholinesterase-positive neurons of mouse islets transplanted to the kidney

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Collagenase-isolated pancreatic islets from C57BL/6J mice were cultured overnight and transplanted under the kidney capsule of non-diabetic syngeneic hosts. Cryostat sections of grafts and fresh islets were stained for acetylcholinesterase (AChE) and vasoactive intestinal polypeptide-like immunoreactivity (VIP-LI). Immediately after isolation, as well as 2–5 days after transplantation, VIP-LI- and AChE-positive nerve cell bodies were clearly seen in the periphery of the islets. Grafts 3–5 days old exhibited a transient and marked increase in VIP-LI nerve cell bodies and fibers. Seven days after transplantation VIP-LI nerve structures began to decrease in number and after 26–52 weeks they were no longer detectable. In contrast, AChE-positive nerve cell bodies and fibers, which showed a relatively constant pattern of distribution, were observed throughout the entire observation period. Restaining experiments demonstrated the coexistence of VIP-LI and AChE activity in the neurons. It is concluded that the grafts were extensively equipped with an intrinsic VIP-ergic and AChE-positive innervation. The initial, transient enhancement of VIP-LI expression probably reflects an adaptation of the neuro-insular complex to the preganglionic denervation, or to the ectopic environment, or

著录项

来源
《cell and tissue research》 |1996年第3期|391-400|共页
作者
SolveigPersson-Sjögren; StureForsgren; PålRoothInge-BertTäljedal;
展开▼
作者单位

Department of Histology and Cell Biology, Umeå University, S-901 87 Umeå, Sweden;

Department of Anatomy, Umeå University, S-901 87 Umeå, Sweden;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类
关键词

Initial increase and subsequent loss of vasoactive intestinal polypeptide immunoreactivity in acetylcholinesterase-positive neurons of mouse islets transplanted to the kidney

摘要

著录项

相关主题

期刊订阅