Calibration method of tilt and azimuth angles for alignment of TEM tomographic tilt series

Misa Hayashida; Shinya Terauchi; Toshiyuki Fujimoto

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Calibration method of tilt and azimuth angles for alignment of TEM tomographic tilt series

机译：Calibration method of tilt and azimuth angles for alignment of TEM tomographic tilt series

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摘要

This paper describes the calibration method of the tilt and azimuth angles of specimen using a digital protractor and a laser autocollimator for alignment of electron tomography. It also suggests an easy method to check whether the specimen is tilted by 180.0 deg, and whether the azimuth angle is 0.0 deg; the method involves the use of two images of a rod-shaped specimen collected before and after a 180.0 deg tilt. The method is based on the assumption that these images are symmetric about the tilt axis when the azimuth angle is 0.0 deg. In addition, we used an experiment to demonstrate the effect of the incorrect angles on reconstructed images and simulated the image quality against distance away from tilt axis.

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来源
《Review of Scientific Instruments》 |2011年第10期|103706-1-103706-6|共6页
作者
Misa Hayashida; Shinya Terauchi; Toshiyuki Fujimoto;
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作者单位

National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, Central 5, Higashi 1-1-1, Tsukuba, Ibaraki 305-8565, Japan;

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正文语种英语
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Calibration method of tilt and azimuth angles for alignment of TEM tomographic tilt series

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