Structural and functional characterization of the two phosphoinositide binding sites of PROPPINs, a β-propeller protein family

Krick R.; Busse R.A.; Scacioc A.Stephan M.Janshoff A.Thumm M.Kühnel K.

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Structural and functional characterization of the two phosphoinositide binding sites of PROPPINs, a β-propeller protein family

机译：Structural and functional characterization of the two phosphoinositide binding sites of PROPPINs, a β-propeller protein family

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β-propellers that bind polyphosphoinositides (PROPPINs), a eukaryotic WD-40 motif-containing protein family, bind via their predicted β-propeller fold the polyphosphoinositides PtdIns3P and PtdIns(3,5) P_2 using a conserved FRRG motif. PROPPINs play a key role in macroautophagy in addition to other functions. We present the 3.0-?crystal structure of Kluyveromyces lactis Hsv2, which shares significant sequence homologies with its three Saccharomyces cerevisiae homologs Atg18, Atg21, and Hsv2. It adopts a seven-bladed β-propeller fold with a rare nonvelcro propeller closure. Remarkably, in the crystal structure, the two arginines of the FRRG motif are part of two distinct basic pockets formed by a set of highly conserved residues. In comprehensive in vivo and in vitro studies of ScAtg18 and ScHsv2, we define within the two pockets a set of conserved residues essential for normal membrane association, phosphoinositide binding, and biological activities. Our experiments show that PROPPINs contain two individual phosphoinositide binding sites. Based on docking studies, we propose a model for phosphoinositide binding of PROPPINs.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2012年第30期|E2042-E2049|共8页
作者
Krick R.; Busse R.A.; Scacioc A.Stephan M.Janshoff A.Thumm M.Kühnel K.;
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作者单位

Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D-37077 G?ttingen, Germany;

Institute of Physical Chemistry, Georg August University, D-37077 G?ttingen, Germany;

Department of Biochemistry II, Georg August University, D-37073 G?ttingen, Germany;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Autophagy; Protein-lipid interactions; X-ray crystallography; Yeast;

Structural and functional characterization of the two phosphoinositide binding sites of PROPPINs, a β-propeller protein family

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