ABSTRACTHistamine was determined by reversed‐phase high‐pressure liquid chromatography in trichloroacetic acid extracts after derivatization with o‐phthaladehyde. Fluorescence was monitored at 350 nm excitation and 450 nm emission wavelength after elution with water containing 40 acetonitrile. The advantage of the described method is a rapid analysis in an automated system, where no selective extraction procedure is necessary and interfering substances are easily separated from the histamine fluoro
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