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Diversity and biodeteriorative potential of fungal dwellers on ancient stone stela

机译:古代石碑上真菌种群的多样性和生物降解潜力

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Biodeterioration caused by fungal colonizers on an ancient stone stela, excavated from the fortner Roman settlement (Eastern Serbia) was investigated. According to selected biodeterioration elements, average deterioration index was assessed (0.8), prompting the need for conservation. Fungal somatic and reproductive structures, along with lichen thalli and moss fragments, were detected on the surface using different microscopy and cultivation methods. In situ microscopy on the site was implemented, for the first time, in the study of stone monuments. Biodeteriorative potential of 5 selected isolates was tested using qualitative biochemical tests, SEM-EDS and XRPD analyses. Fusarium proliferatum and Penicillium crustosum altered the pH value in broth minimal medium. Pigment production was demonstrated for F. proliferatum, while P. crustosum showed potential for calcite dissolution. All isolates induced biomineralization on solid medium with calcium acetate, where weddellite, calcite and subordinate whewellite crystals were confirmed via SEM-EDS and XRPD. Weddellite and calcite production was documented for P. crustosum in solid medium with calcium carbonate. Conservation treatment was carried out with benzalkonium chloride-based biocide, in addition to mechanical treatment. After conservation, in situ microscopy showed deteriorated stone surface covered with residual lichen thalli fragments, while mycelium and reproductive structures of micromycetes were not detected. (C) 2016 Elsevier Ltd. All rights reserved.
机译:研究了真菌定居者在古罗马石碑上造成的生物退化,石碑是从罗马要塞定居点(东塞尔维亚)发掘的。根据选定的生物恶化因素,评估了平均恶化指数(0.8),这提示需要进行保护。使用不同的显微镜和培养方法在表面上检测到真菌的体细胞和生殖结构以及地衣藻和苔藓碎片。现场首次在石碑研究中实施了现场显微镜检查。使用定性生化测试,SEM-EDS和XRPD分析测试了5个选定菌株的生物降解潜力。镰刀菌和青霉改变了肉汤基本培养基中的pH值。已证明增生镰刀菌的色素生产,而克鲁氏假单胞菌显示方解石溶解的潜力。所有分离物均在固体培养基上用乙酸钙诱导生物矿化,其中通过SEM-EDS和XRPD证实了闪锌矿,方解石和次要的辉绿岩晶体。在碳酸钙固体培养基中记载了克鲁氏假藻的Weddellite和方解石生产。除机械处理外,还使用基于苯扎氯铵的杀菌剂进行保护处理。保存后,原位显微镜检查显示,腐烂的石头表面覆盖有残留的地衣藻碎片,而未检测到菌丝体和菌丝的生殖结构。 (C)2016 Elsevier Ltd.保留所有权利。

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