Engineering RNA sequence specificity of Pumilio repeats

Cheong  CG; Hall  TMT

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Engineering RNA sequence specificity of Pumilio repeats

机译：Engineering RNA sequence specificity of Pumilio repeats

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Puf proteins bind RNA sequence specifically and regulate translation and stability of target mRNAs. A "code" for RNA recognition has been deduced from crystal structures of the Puf protein, human Pumilio1, where each of eight repeats binds an RNA base via a combination of three side chains at conserved positions. Here, we report the creation of seven soluble mutant proteins with predictably altered sequence specificity, including one that binds tightly to adenosine-uracil-rich element RNA. These data show that Pumilio1 can be used as a scaffold to engineer RNA-binding proteins with designed sequence specificity.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2006年第37期|13635-13639|共5页
作者
Cheong CG; Hall TMT;
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作者单位

Natl Inst Environm Hlth Sci, Lab Struct Biol, NIH, Res Triangle Pk, NC 27709 USA;

p3.inet.fi;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
protein design; Puf proteins; protein-RNA interaction; adenosine-uracil-rich elements; GERMLINE STEM-CELLS; HUNCHBACK MESSENGER-RNA; 3' UNTRANSLATED REGION; STRUCTURE-BASED DESIGN; BINDING PROTEINS; CAENORHABDITIS-ELEGANS; DROSOPHILA EMBRYOS; DEPENDENT CONTROL; HOMOLOGY DOMAIN; ZINC FINGERS;

Engineering RNA sequence specificity of Pumilio repeats

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