A compact fiber-optic SHG scanning endomicroscope and its application to visualize cervical remodeling during pregnancy

Zhang Y.; Akins M.L.; Murari K.Xi J.Li M.-J.Luby-Phelps K.Mahendroo M.Li X.

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A compact fiber-optic SHG scanning endomicroscope and its application to visualize cervical remodeling during pregnancy

机译：A compact fiber-optic SHG scanning endomicroscope and its application to visualize cervical remodeling during pregnancy

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摘要

We report the development of an all-fiber-optic scanning endomicroscope capable of high-resolution second harmonic generation (SHG) imaging of biological tissues and demonstrate its utility for monitoring the remodeling of cervical collagen during gestation in mice. The endomicroscope has an overall 2.0 mm diameter and consists of a single customized double-clad fiber, a compact rapid two-dimensional beam scanner, and a miniature compound objective lens for excitation beam delivery, scanning, focusing, and efficient SHG signal collection. Endomicroscopic SHG images of murine cervical tissue sections at different stages of normal pregnancy reveal progressive, quantifiable changes in cervical collagen morphology with resolution similar to that of bench-top SHG microscopy. SHG endomicroscopic imaging of ex vivo murine and human cervical tissues through intact epithelium has also been performed. Our findings demonstrate the feasibility of SHG endomicroscopy technology for staging normal pregnancy, and suggest its potential application as a minimally invasive tool for clinical assessment of abnormal cervical remodeling associated with preterm birth.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2012年第32期|12878-12883|共6页
作者
Zhang Y.; Akins M.L.; Murari K.Xi J.Li M.-J.Luby-Phelps K.Mahendroo M.Li X.;
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作者单位

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, United States;

Department of Obstetrics and Gynecology, UT Southwestern Medical Center, Dallas, TX 75390, United States;

Science and Technology Division, Corning Incorporated, Corning, NY 14831, United StatesDepartment of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390, United States;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Cervical collagen remodeling; Fiber-optic scanning probe; Nonlinear endomicroscopy; Parturition;

A compact fiber-optic SHG scanning endomicroscope and its application to visualize cervical remodeling during pregnancy

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